PCR Inhibition Assay for DNA-targeted Antibiotics.
نویسندگان
چکیده
منابع مشابه
PCR inhibition assay for DNA-targeted antibiotics.
DNA amplification by polymerase chain reaction (PCR) should be inhibited if the target for amplification region in the template DNA is nicked or cut. Based on this premise, we established a sensitive and differential assay using PCR to detect antibiotics that act on DNA. After template lambda DNA (10 pg) was incubated with antibiotics (10 approximately 20 ng) at 37 degrees C for 30 minutes in a...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1995
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.48.1267